Target of this antibody:
Alternative names of antibody target:
CXCR5 , MDR15 , CD185 , C-X-C chemokine receptor type 5 , BLR1
Chemokines play an important role in inflammation and critical for the recruitment of effector immune cells to sites of infection. Chemokines activate leukocytes by binding to G protein coupled receptors. The ever-growing chemokine receptor subtypes can be divided into 2 major groups, CXCR and CCR, based on the 2 major classes of chemokines. CXCR5 is one of the CXCR receptors, which is expressed in a variety of cells.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide surrounding amino acid 359 of human CXCR5
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry
Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 36 kDa CXCR5 of human origin. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.