Target of this antibody:
Alternative names of antibody target:
Protein lin-41 homolog
TRIM71 is similar to the C.elegans homolog protein Lin41. TRIM71 is a target of the microRNA let-7 and is likely to play a role in mammalian development. From recent research, TRIM71 has been indicated as a ubiqutin ligase that can interact with Dicer and Argonaut proteins Ago1, Ago2 and Ago4.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 231of mouse TRIM71
Concentration of antibody:
Form of antibody:
Affinity Purified Antibody
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-TRIM71 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western Blot analysis (2-4 µg/ml). However, the optimal concentrations should be determined individually. Blocking peptide (Cat.# 3241BP-50) is available separately.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.