anti-p53 Antibody (Clone C6A5)

Catalog number
3036-100
Name
anti-p53 Antibody (Clone C6A5)
Size
p53 Antibody (Clone C6A5)
Price
371.00 EUR
Supplier

Details

Target of this antibody:
p53
Alternative names of antibody target:
TP53, TRP53, P53, LFS1, Tumor suppressor p53, Phosphoprotein p53
Background information:
p53, a 53 kDa protein, binds to a DNA consensus sequence, the p53 response element, and regulates normal cell cycle events by activating transcription of genes involved either in progression throμgh the cell cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the concentration in normal cells, principally due to the increased half-life (4 hrs) compared to that of wild-type (20 min). p53 localizes in the nucleus, but is detectable at the plasma membrane during mitosis and certain mutations also modulate cytoplasmic/nuclear distribution. p53 downregulates Bcl-2 expression and upregulates Bax expression, but may not always be necessary for apoptosis.
Antibody host:
Mouse
Clonality of antibody:
Monoclonal
Isotype:
Mouse IgG1
Clone:
C6A5
This antibody reacts with:
Human, mouse, rat
Immunogene sequence:
Synthetic peptide corresponding to residue surrounding Ser20 of human p53
Concentration of antibody:
0.5 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
100 µg (0.5 mg/ml) in PBS containing 1 mg/ml BSA and 1.5 mM sodium azide and 50% glycerol.
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
Recommended usage:
Western blotting (0.5-4 µg/ml), immunoprecipitation (4-8 µg/ml), Immunohistochemistry (4-8 µg/ml), immunocytochemistry (4-8 µg/ml). However, the optimal conditions should be determined individually.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps