Target of this antibody:
Alternative names of antibody target:
N6-methyladenosine (m6A), or methylation of the N6 position of adenosine is a posttranscriptional modification of RNA. Due to a lack of analytical methods, N6-methyladenosine is poorly understood, but new evidence suggests that it is a very common base modification and important physiological regulator. N6-methyladenosine is markedly increased throughout brain development, and is enriched near stop codons, microRNA-binding sites and UTRs, which indicates a fundamental role in the regulation of gene expression. N6-methyladenosine is also highly conserved between human and mouse. The regulation of m6A modifications in mRNA has been linked to disease, where fat mass and obesity-associated (FTO) has been has been reported to be a obesity risk gene. FTO is a m6A demethylase and polymorphisms that result in increased FTO expression are associated with increased body mass and risk of obesity.
Clonality of antibody:
This antibody reacts with:
This antibody is generated from rabbits immunized with a BSA conjugated N6-Methyladenosine (BV-N13-14).
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
30 µg (1 mg/ml) in PBS (prepared using DEPC-treated water) with 0.09% (W/V) sodium azide.
Protein A and immunogen affinity purified antibody
This antibody has been succesfully tested for use in following methods:
Western blot, Dot Blot, Chromatin Immunoprecipitation (ChIP), RNA Binding Protein Immunoprecipitation (RIP).
Western blot: 1-4 µg/ml. Dot Blot, Chromatin Immunoprecipitation (ChIP), RNA Binding Protein Immunoprecipitation (RIP).
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.