ABTS™ for cell activation

Catalog number
1212-100
Name
ABTS™ for cell activation
Size
100 ml
Price
153.00 EUR
Supplier

Details

Description
ABTS is considered a safe and sensitive substrate compatible for horseradish peroxidase (HRP) based ELISA assays. In the presence of hydrogen peroxide and HRP, ABTS is oxidized to a radical cation with an adsorption maxima at 820 nm, 734 nm, 650 nm and 405 nm. The latter frequency demonstrates a significant molar extinction coefficient and is generally employed for most ABTS assays. Stopping the reaction with acid does not alter the 405 nm spectrum allowing kinetic and endpoint methods to be measured at the same wavelength. Using a proprietary-nontoxic stabilization technology, BioVision, Inc. provides a room temperature stable, single component ABTS solution for quantitative analysis of HRP based systems.
Peptide sequence
N/A
CAS number
30931-67-0
Molecular weight
514.6
Category
Biochemicals
Other name
2,2'-Azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid)
Molecular formula
C₁₈H₁₈N₄O₆S₄
Physical appearance
Liquid
Supplied with
1.46 mMol L⁻¹, in a citrate buffer, pH 4.0.
Is this a salt?
No
Is it cell-permeable?
No
Purification
N/A
Reconstitute instructions
N/A
Storage condition
+4°C
Shipping condition
RT
Maximum time for storage
12 months
Storage instructions
Protect from light
Tissue
cell
Additional description
For cells, cell lines and tissues in culture till half confluency.