Target of this antibody:
Alternative names of antibody target:
PARP1, ADPRT, PPOL, NAD(+) ADP-ribosyltransferase 1
PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215, which separates PARP’s N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide corresponding to caspase cleavage site in human PARP
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
30 µg (0.2 mg/ml) affinity purified rabbit anti-PARP polyclonal antibody in phosphatebuffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot analysis (1-4 µg/ml). The polyclonal anti-PARP antibody recognizes the full length (116 kDa) and the cleaved fragment (85 kDa) of human PARP. However, the optimal concentrations should be determined individually.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.