Target of this antibody:
Alternative names of antibody target:
PAK1, PAK-1, p21/Cdc42/Rac1-activated kinase 1. PAK alpha, Alpha-PAK , p65-PAK, MGC130001, MGC130000
Human p21-GTPase-activated protein kinase 1 (PAK1) is a functional homolog of STE20 of Saccharomyces cerevisiae. PAK1 is a downstream effector of the Rho-family small GTPases Rac and Cdc42. A serine/threonine kinase, PAK1 induces the formation of filopodia and lamellipodia in Swiss 3T3 cells. PAK1 inhibits myosin light chain kinase, and appears to play a role in the regulation of apoptosis by phosphorylating the death agonist Bad. Membrane targeting of PAK1 induces neurite outgrowth. PAK1 may also regulate NFkB activation.
Clonality of antibody:
This antibody reacts with:
Recombinant human PAK1
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.2 mg/ml) Protein A affinity purified rabbit anti-PAK1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation
Western blotting (0.5-4 µg/ml) and immunoprecipitation (10-20 µg/ml). However, the optimal conditions should be determined individually.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.