Target of this antibody:
Alternative names of antibody target:
LIM kinases, LIMK-1
LIM kinases (LIMK1 and LIMK2) are serine/threnine kinases that have two zinc finger motifs, known as LIM motifs in their N-terminal regulatory domain. LIM kinases are involved in actin cytoskeleton regulation throμgh Rho-family GTPases and downstream kinases PAKs and ROCK. PAK1 and ROCK phosphorylate LIMK1 and LIMK2, which increases the activity of the kinases. Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the N-terminus of Cofilin.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide corresponding to the C-terminus of human LIMK1
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) antigen affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation
Western blotting (1-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 70 kDa LIMK1 protein. It does not cross-react with LIMK2.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.