Target of this antibody:
Alternative names of antibody target:
SMAP45 , HD3 , RPD3 , RPD3-2
HDAC3 is a member of the class I mammalian histone deacetylase family, which plays an important role in modulating the eukaryotic chromatin structure. Human HDAC3 is composed of 428 amino acid residues. HDAC3 interacts with a growing number of transcriptional factors including SMRT and N-CoR. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat, chicken
Synthetic peptide corresponding to the C-terminus of human HDAC-3
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.2 mg/ml) peptide affinity purified rabbit anti-HDAC3 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry
Western blotting (0.5-4 µg/ml)and Immunohistochemistry (20-30 µg/ml). However, the optimal conditions should be determined individually.The antibody detects the 49 kDa HDAC3. It does not cross-react with other HDAC proteins including HDAC1, 2, 4, 5, 6, 7, and 8. Jurkat cell lysate (Cat.# 2401-100) can be used as a positive control.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.