Target of this antibody:
Alternative names of antibody target:
P-cadherin, N-Cadherin, E-Cadherin, K-Cadherin, M-jadherin, R-Cadherin
Cadherins comprise a family of Ca-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Cadherins consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of cadherins is thoμgh to be responsible for binding specificity, transmembrane domains and carboxy terminal domains. The relative short intracellular domains interact with a variety of cytoplasmic proteins, such as β-catenin, to regulate cadherin function.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide surrounding amino acid 388 of human Cadherin
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-cadherin polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Immunohistochemistry
Western blotting (1-2 µg/ml), immunoprecipitation (10-20 µg/ml), and Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects E- and P-cadherin (~95 kDa). Jurkat cell lysate (Cat.# 2401-100) can be used as a positive control.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.