• CONTENTS: Supplied as a 50% slurry in 20% ethanol/ H₂O;> 3 mg (10 µmol) glutathione per ml Sepharose beads. • FEATURES: The Glutathione-S-Transferase (GST) gene fusion system has been widely used for the over expression of foreign genes in E. coli. The expressed fusion protein with a GST tail can be easily purified by affinity chromatography on Glutathione-Sepharose beads from the bacterial lysate. BioVison’s Glutathione-Sepharose is designed for the purpose of purification of such GST fusion proteins or any other kinds of glutathione binding proteins. This formulation exhibits excellent binding capacity, high flow rate, no significant loss of the glutathione ligand and a pH stability range of 2-10. • APPLICATIONS: Purification of GST-fusion proteins or other glutathione-binding proteins.
Glutathione-Sepharose is prepared by covalently coupling glutathione to epoxy-activated 4 % cross-linked sepharose beads to form a stable thioether linkage. The coupling was optimized to give a high binding capacity and could be greater than 10 mg of Glutathione-S-transferase (GST) per ml of wet gel.
Maximum time for storage