Target of this antibody:
Alternative names of antibody target:
CBP-tag, Calmodulin binding peptide-tag
Calmodulin binding peptide (CBP) tag is a fusion protein that is useful for immunoaffinity purification of proteins expressed in E.coli. The CBP tag binds to calmodulin resin in the presence of low concentration of calcium and can be eluted with a neutral buffer. The antibody is suitable for identifying CBP-fusion proteins.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
A synthetic CBP tag peptide KRRWKKNFIAVSAA conjμgated to KLH.
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
30 µg (0.5 mg/ml) affinity purified rabbit anti-CBP-Tag polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.5 mM EDTA and 0.03% ProClin®.
This antibody has been succesfully tested for use in following methods:
The antibody can be used for Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Blocking peptide (Cat.# 3960BP-50) is available separately.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.