anti-MAPKAPK-2 Antibody

Catalog number
3100-100
Name
anti-MAPKAPK-2 Antibody
Size
MAPKAPK-2 Antibody
Price
338.00 EUR
Supplier

Details

Target of this antibody:
MAPKAPK-2
Alternative names of antibody target:
MAPKAPK2, MK2
Background information:
MAP kinase-activated protein kinase-2 (MAPKAPK-2), also known as p45 hsp27 kinase, is a 45-54 kDa serine-threonine protein kinase that contains a proline rich sequence and two putative SH3-binding sites. MAPKAPK-2 is activated in response to stress, IL-1 and TNF, possibly catalyzed by p38/Hog-dependent phosphorylation. One of the major substrates of MAPKAPK-2 is hsp27, which stimulates actin polymerization in order to facilitate recovery from destruction of cytoskeleton during cellular stresses. Two isoforms are produced due to alternative splicing of the same gene which differs in their C-terminals. This antibody recognizes both isoforms.
Antibody host:
Rabbit
Clonality of antibody:
Polyclonal
Isotype:
Rabbit IgG
This antibody reacts with:
Human, mouse, rat, dog, bovine
Immunogene sequence:
Synthetic peptide surrounding amino acid 365 of mouse MAPKAPK-2
Concentration of antibody:
0.5 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-MAPKAPK-2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation
Recommended usage:
Western blot analysis (0.5-4 µg/ml) and immunoprecipitation. However, the optimal conditions should be determined individually. The affinity purified antibody detects both 43 and 60 kDa isoforms, corresponding to the apparent molecular mass of MAPKAPK-2 on SDS-PAGE immunoblots. These 43 kDa and 60 kDa bands can be specifically inhibited by the relevant peptide.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps