Target of this antibody:
Alternative names of antibody target:
CD40LG, CD154, TRAP , gp39 , # CD40L , IMD3 , IGM , hCD40L, TNFSF5. HIGM1, CD40-L
CD40, also known as bp50 or CDW40, is a type I membrane protein found on the surface of B cells and primary carcinomas. It belongs to the TNF-R family. CD40 serves as the receptor for CD40 ligand (CD40L), a cytokine expressed on the cell surface of T cells. CD40 plays a critical role in B cell proliferation, antibody class switching, modulation of apoptosis in the germinal center throμgh interaction with T cells expressing CD40L, and activation of CD4+ T cells. Ligation of CD40 with its ligand preferentially induces stress-activated protein kinases in B lymphocytes.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 246 of human CD40 Ligand
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-CD40L polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Jurkat cell lysate can be used as a positive control.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.