Target of this antibody:
Alternative names of antibody target:
BIRC5, IAP4 , API4, SVV , EPR-1
Survivin is a newly described apoptosis inhibitor that is expressed in many human cancers, but undetectable in terminally differentiated adult tissues. It has been shown that recombinant expression of Survivin counteracts apoptosis of B lymphocyte precursors deprived of interleukin 3 (IL-3), sμggesting a potential role of Survivin in cancer therapy. Survivin is expressed in G2-M phase in a cell cycle-dependent manner and directly associated with mitotic spindle microtubules, sμggesting a role in both apoptosis regulation and cell cycle progression.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide surrounding amino acids near the C-terminus of human survivin
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-Survivin polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunofluorescence
Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.