Target of this antibody:
Alternative names of antibody target:
DANTE, CKTSF1B3, DAN domain family member 5, Cerberus-like 2 protein
COCO, also known as DAND5, Dante, and CKTSF1B3, is a member of the DAN domain family of BMP antagonists that includes DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), and Cerberus (DAND4). DAN family members contain a cysteine knot domain that is homologous to that found in other TGFβ superfamily ligands such as BMPs that play important roles in tissue morphogenesis and developmental processes. COCO has eight Cys residues in the cysteine knot which places it in the CAN (or eightmembered ring) subfamily of BMP antagonists along with the other DAN family proteins. In Xenopus embryos, COCO is expressed by pluripotent ectodermal cells. Expression is abruptly downregulated prior to gastrulation, and the loss of ectodermal cell pluripotency is coincident with COCO downregulation. COCO is required for Xenopus leftright axis formation. It functions predominantly on the right side of the embryo, although it is equally expressed on both left and right sides. COCO binds and inhibits activin, BMP4, GDF3/derrière, Wnt8, and Xnr1. In mouse, COCO expression is elevated on the right side of Henson’s node at the early somite stage, in contrast to the left side expression of Nodal.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide at C-terminal (BV-MM26)
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) of antibody in PBS containing 0.01 % BSA, 0.01 % thimerosal, and 50 % glycerol, pH 7.2.
This antibody has been succesfully tested for use in following methods:
Western blot: 1:200
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.