Target of this antibody:
Alternative names of antibody target:
DKK-3, DKK 3, dkk3, dkk-3, dkk 3, dickkopf homolog-3, dickkopf homolog3, dickkopf homolog 3
Xenopus Dickkopf (Dkk) was initially discovered as a Wnt antagonist that plays an important role in head formation. By far, four members of Dkk have been identified in mammals. Each Dkk molecule contains two conserved cysteine-rich domains which are separated by a 50-55 amino acid linker region.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide surrounding amino acid 240 of rat Dkk3
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) antigen affinity purified rabbit anti-Dkk3 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol and 0.01% Thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~38 kDa of Dkk3 in samples from mouse and rat origins. Reactivity to other species has not been determined.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.