Target of this antibody:
Alternative names of antibody target:
Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-11 is also synthesized as inactive pro-enzyme that are processed in cells undergoing apoptosis or inflammation. The processed form of caspase-11 consists of large and small subunits which associate to form an active enzyme.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide surrounding amino acid 257 of rat caspase-11
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
30 µg (0.2 mg/ml) protein A purified rabbit anti-caspase-11 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml). The antibody detects procaspase-11 (45 kDa), a partially cleaved fragment (30 kDa) and fully cleaved fragment (20 kDa). A 58 kDa unknown identity band was also detected in 3T3 cells.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.