Target of this antibody:
Alternative names of antibody target:
Cell adhesion molecules, BL-CAM ,SIGLEC-2 , Siglec-2, SIGLEC2, CD22, FLJ22814 , MGC130020, Leu-14,
Cell adhesion molecules (CAMs) are a family of closely related cell surface glycoproteins involved in cell-cell interactions during growth and are thoμght to play an important role in embryogenesis and development. BLCAM α and BLCAM β are produced by alternative splicing of the CD22 gene and form a heterodimer that mediates B cell-B cell interactions.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 85 of human BLCAM
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.2 mg/ml) affinity purified rabbit anti-BLCAM polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Immunohistochemistry
Western blotting (0.5-4 µg/ml), immunoprecipitation (5-10 µg/ml), and Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.