DOT1L (2- 416 aa), Human recombinant

Catalog number
7666-25
Name
DOT1L (2- 416 aa), Human recombinant
Size
25 μg
Price
295.00 EUR
Supplier

Details

Target
DOT1L
Background
Dot1L is a non-SET domain containing methyltransferase. It is the only enzyme known to methylate histone 3 at lysine 79, where it catalyzes mono-, di-, and trimethylation. Proper Dot1L function is necessary for transcriptional activation of many genes, DNA damage repair, and cell cycle regulation. Dot1L plays an essential role in MLL-rearranged leukemias and is being investigated as a drug-target for these leukemias. Experiments using siRNA or shRNA have shown that knockdown of Dot1L leads to loss of proliferation in lung cancer cells or faster reprogramming and increased yield of induced pluripotent stem cells, respectively. This protein product contains the methyltransferase domain of Dot1L.
Concentration
N/A
Molecular weight
74.1 kDa (2- 416 aa + NT GST Tag)
Synonym
DOT1L (2- 416 aa), Human recombinant
Other name
Dot1-like; Disruptor of Telomeric Signaling 1-Like
NCBI gene number
84444
NCBI gene
DOT1L
Gene source
Human
NCBI number
Q8TEK3
Recombinant
Yes
Source
E. coli
Purification
≥95%
Tested applications
SDS-PAGE, HPLC
Level of endotoxin
N/A
Tested activity
N/A
Biological activity
N/A
Reesults
N/A
Binding ability
N/A
Unit
N/A
Storage condition
-80°C
Shipping under
dry ice
Physical appearance
Liquid
Physical properties
20 mM Tris, pH 8.0, containing 200 mM sodium chloride, 1 mM EDTA and 1 mM DTT.
How to reconstitute
N/A
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants