anti-PARP Antibody (Clone 10H)

Catalog number
3000-100
Name
anti-PARP Antibody (Clone 10H)
Size
PARP Antibody (Clone 10H)
Price
371.00 EUR
Supplier

Details

Target of this antibody:
PARP
Alternative names of antibody target:
PARP1, ADPRT, PPOL, NAD(+) ADP-ribosyltransferase 1
Background information:
PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215, which separates PARP’s N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.
Antibody host:
Mouse
Clonality of antibody:
Monoclonal
Isotype:
Mouse IgG3κ
Clone:
10H
This antibody reacts with:
All species
Concentration of antibody:
1.0 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
1 mg/ml of IgG purified from mouse ascites by protein A chromatography. Prepared in 20 mM Tris, pH 7.4, 150 mM NaCl, 1% BSA and 0.02% sodium azide.
Purification method:
Chromatography
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Immunocytochemistry & ELISA
Recommended usage:
Immunocytochemistry : 5-20 μg/ml; Western Blot (10 μg/ml using colorimetric methods;
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps