Target of this antibody:
Alternative names of antibody target:
VDAC1, VDAC-1 , MGC111064 , hVDAC1, Porin
The Voltage-Dependent Anion Channel (VDAC or mitochondrial Porin)I is an outer membrane mitochondrial protein. The VDAC protein is thoμght to form the major pores throμgh which adenine nucleotides are transferred throμgh the outer mitochondrial membrane. VDAC has also been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, ANT, and CypD is thoμght to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat, bovine, rabbit
Synthetic peptide surrounding amino acid 189 of human VDAC
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-VDAC/Porin polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry
Western blotting (0.5-4 µg/ml) and Immunohistochemistry (2.5 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~31 kDa VDAC/Porin from samples of human, mouse, rat, bovine, pig, and rabbit origins.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.