Target of this antibody:
Alternative names of antibody target:
Protein kinase C
PKC (Protein kinase C) is a calcium-dependent and phospholipid-dependent enzyme that is activated in vivo by the lipid diacylglycerol. PKC has been shown to be involved in signal transduction and various aspects of neoplastic transformation. PKC contains an amino terminal regulatory domain and a carboxy terminal catalytic domain joined by a hinge region. Cleavage of protein kinase C in vitro results in the generation of two functional fragments corresponding to these two domains.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic phospho-peptide surrounding amino acid Ser660 of human PKC beta II
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide.
This antibody has been succesfully tested for use in following methods:
The antibody can be used in Western blotting (1-2 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes several Phosphorylated PKC (Ser660) isoforms of human, mouse, and rat origins. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.