Target of this antibody:
Alternative names of antibody target:
TMS1, hASC, PYCARD, CARD5, MGC10332
ASC (also called TMS1) is a CARD domain containing protein. ASC induces apoptosis throμgh activation of caspase-9 and inhibiting the survival of human breast cancer cells. Overexpression of ASC/TMS1 induced DNA fragmentation. ASC/TMS1 is expressed in a variety of human and mouse tissues.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide corresponding to residues surrounding amino acid 185 of human ASC
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-ASC/TMS1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The affinity purified antibody detects the 25 kDa human and rat ASC/TMS1. HL60 cell lysate can be used as a positive control.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.