Target of this antibody:
Alternative names of antibody target:
Members of the Bcl-2 family of proteins interact to regulate programmed cell death, or apoptosis. Various homodimers and heterodimers formed by proteins in this family can either promote or inhibit apoptosis. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect on certain hematopoietic cell lines following growth factor withdrawal. Additional apoptotic inhibitors in this family include Bcl-x, Bcl-w, Mcl-1, Bag-1 and A1. Pro-apoptotic members of this family include Bax, Bad, Bak, NBK (Bik), BID and Hrk. Hrk (for harakiri), designated DP5 or neuronal death protein in mouse and rat, contains a BH3 domain with high homology to other Bcl-2 family members but lacks the conserved BH1 and BH2 domains. Physical interaction of Hrk with Bcl-2 or Bcl-xL inhibits the apoptotic activity of Hrk.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide corresponding to residues surrounding amino acids 50 of human HRK
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) antigen affinity purified rabbit anti-human Hrk polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.
This antibody has been succesfully tested for use in following methods:
Western blot analysis (1 µg/ml). However, the optimal conditions should be determined individually. The immunoaffinity purified antibody detects human Hrk fused with green fluorescent protein in crude extracts of E. coli transfected with Hrk-GFP.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.