Target of this antibody:
Alternative names of antibody target:
TNFSF10, TRAIL-PEN, TL2, Apo-2L, CD253, APO2L
TRAIL (TNF-related apoptosis-inducing ligand), a member of the TNF ligand family, is a type II membrane protein and expressed in a variety of human tissues. Two death domains containing receptors DR4 and DR5 have been identified as the receptors for TRAIL and two decoy receptors designated DcR1 and DcR2 inhibit TRAIL function. The multiple receptors sμggest a complex role for regulation of signaling by the cytokine. TRAIL induces apoptosis and NF-kB activation in many tissues and cells.
Clonality of antibody:
This antibody reacts with:
E. coli expressed recombinant human TRAIL
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified, rabbit anti-TRAIL polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.
This antibody has been succesfully tested for use in following methods:
Western blot, Neutralization
Western blot analysis (0.5-1 µg/ml), ELISA (2-3 µg/ml) and neutralization (5-10 µg/ml). However, the optimal conditions should be determined individually.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.