Target of this antibody:
Alternative names of antibody target:
UNQ421/PRO3674, ECSM4, FLJ20798, MGC133352, MGC133353, MRB
ROBO4 contains two fibronectin type-III domains and two Ig-like C2-type domains. ROBO4 is predominantly expressed in vascular endothelium and is considered important for vascular development. ROBO4 is a receptor for Slit proteins and may mediate the inhibition of primary endothelial cell migration by Slit proteins.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide surrounding amino acid 322 of rat ROBO4
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti- ROBO4polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (1-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects the ROBO4 band in human samples.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.