Target of this antibody:
Alternative names of antibody target:
Transforming growth factor beta-1, TGF-beta-1
TGF-β(transforming growth factor-beta) is a family of multifunctional proteins. TGF-β was originally identified for its ability to induce the growth of normal rodent fibroblast in soft agar. It is now known that TGF-β is a potent growth inhibitor of many normal and transformed cell lines. It regulates normal cell growth, development and tissue remodeling following injury. TGF-β is produced as latent high molecular weight complexes. The activation of latent TGF-β is an important step in the regulation of its action.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat, porcine
Synthetic peptide surrounding amino acid 374 of human TGF-β1.
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified, rabbit anti-TGF-β1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
The antibody can be used for Western blot analysis (0.5-4 µg/ml) and immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. Blocking peptide (Cat.# 5559BP-50) is available separately.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.