anti-Hsp70 Antibody (Clone S5)

Catalog number
anti-Hsp70 Antibody (Clone S5)
Hsp70 Antibody (Clone S5)
371.00 EUR


Target of this antibody:
Alternative names of antibody target:
HSPA4, APG2, APG-2, HSP70RY, HS24/P52, MGC131852 , hsp70RY
Background information:
Hsp70, one class of stress proteins, is comprised of multiple members, all of which bind ATP in vitro, but are localized within different intracellular compartments. These include: a) the constitutive Hsc70 (or cognate) present within the cytosol/nucleus; b) the highly stress-inducible Hsp70 present within the cytosol/ nucleus/nucleolus; c) the constitutive glucose regulated 78 kDa (or BiP) protein present within the lumen of the endoplasmic reticulum; and d) the glucose regulated 75 kDa protein present within the mitochondria. Hsp70 is typically not expressed in the cell under normal growth conditions, but is expressed at high levels in the cell experiencing stress. Consequently, detection of Hsp70 using an antibody specific for the inducible form is quite useful in ascertaining whether a stress response has occurred in the cell.
Antibody host:
Clonality of antibody:
Mouse IgG1
This antibody reacts with:
Human, mouse, rat, monkey, hamster, rabbit, bovine
Immunogene sequence:
Recombinant human Hsp70
Concentration of antibody:
0.5 mg/ml
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) in PBS containing 1 mg/ml BSA and 1.5 mM sodium azide and 50% glycerol.
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Immunohistochemistry
Recommended usage:
Western blot analysis (1 µg/ml), immunoprecipitation (5-10 µg/ml), Immunohistochemistry (5-20 µg/ml, frozen & paraffin), and FACS analysis. However, the optimal conditions should be determined individually. The antibody recognizes a 70 kDa protein, corresponding to the apparent molecular weight of the inducible Hsp70 on SDS-PAGE immunoblots. This antibody has no reactivity with the constitutive Hsc70 (Hsp73).
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation