anti-Oct-4 Antibody

MGC22487, OCT3, OTF3, OTF4, Oct4 , POU5F1 , Oct-3

Members of Oct family of transcription factors specifically interact with Octamer motif ATGCAAAT, a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. All of the members of the Oct family contain two highly conserved domains which are separated by 14-26 variable amino acids. These include the POU homeodomain and the POU-specific domain. Both are required for DNA binding and are involved in protein-protein interactions. Evidences indicate that regulation of Oct family transcription factors occurs at the level of phosphorylation.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

Synthetic peptide mapping to the N-terminus of mouse Oct-4

0.5 mg/ml

Liquid

30 µg (0.5 mg/ml) affinity purified rabbit anti-Oct-3/4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.03% ProClin®.

Affinity purified

Western blot, Immunoprecipitation, Immunohistochemistry

Western blotting (0.5-4 µg/ml), immunoprecipitation (20 µg/ml) and Immunohistochemistry (20 µg/ml). However, the optimal conditions should be determined individually. The antibody reacts with 45 kDa Oct-4 (also designated Oct-3A) and in a less extent 33 kDa Oct-3B from human, mouse, and rat samples. Rat kidney tissue lysate can be used as a positive control.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps