anti-Anti-Osteopontin (Clone 14C08)

Catalog number
anti-Anti-Osteopontin (Clone 14C08)
Anti-Osteopontin (Clone 14C08)
338.00 EUR


Target of this antibody:
Alternative names of antibody target:
OPN, BNSP, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin.
Background information:
Human Osteopontin (OPN) is a negatively charged hydrophilic protein of 314 amino acids and is subject to significant post translational modifications. OPN is cleaved by members of the matrix metalloproteinase family (MMP) which results in the generation of N-terminal and C-terminal OPN fragments. The matricellular protein osteopontin binds to cell surface receptors and is secreted into many body fluids including milk, blood and urine, depending on the organ of origin. This makes osteopontin an ideal candidate for being a biomarker as the secreted form is easily obtained in throwaway fluids, and mimics the cellular environment from which it is released. Osteopontin is important in immune responses and inflammation as well as bone generation and remodeling. In autistic children, serum levels of osteopontin are correlated to the severity of disease, probably due to a brain inflammation pattern in these children. In aortic valve sclerosis and stenosis, increased levels of secreted osteopontin are also noted. Osteopontin has also been sμggested as a cancer biomarker, since it is associated with tumor formation, progression and metastasis. In bone and tooth formation osteopontin is known to be a negative regulator of parathyroid hormone-related protein receptor, which induces osteogenesis. Without appropriate levels of osteopontin, bone growth continues unregulated, and leads to specific bone cancers. In short, osteopontin is a strong marker for bone growth, inflammation and certain cancers. The newly exposed SVVYG epitope on the N-terminal fragment has also been shown to participate in cell adhesion.
Antibody host:
OPN Knockout Mouse
Clonality of antibody:
Mouse IgG1κ
This antibody reacts with:
Recognizes native human OPN from purified breast milk. Specific for the MMP cleavage site DSVVYG of human OPN.
Immunogene sequence:
DSVVYG peptide conjμgated to KLH
Concentration of antibody:
1 mg/ml
Form of antibody:
Formulation of this antibody:
1 mg/ml in 0.15 M PBS
Purification method:
Protein A chromatography from in vitro production
This antibody has been succesfully tested for use in following methods:
ELISA - Sandwich and Indirect. Pairs well with Cat # 5422-100.
Recommended usage:
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.