Target of this antibody:
Alternative names of antibody target:
amyloid-b-peptide, amyloid beta peptide, Amyloid beta A4 protein, Alzheimer disease amyloid protein, ABPP, APPI, Cerebral vascular amyloid peptide,
Accumulation of the amyloid-β peptide (Aβ) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer’s disease. The β-amyloid protein precursor (APP) is cleaved by β-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for g-secretase to generate the 4 kDa amyloid-β peptide, which is deposited in the brains of all suffers of Alzheimer’s disease.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide from human APP
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) immunoaffinity purified rabbit anti-APP polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry
Western blot analysis (1-4 µg/ml) and Immunohistochemistry (2-10 μg/ml). However, the optimal conditions should be determined individually. Murine and rat brain tissues can be used as positive controls.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.