Biotin-VDVAD-FMK for cell activation

This is a synthetic peptide that irreversibly inhibits caspase-2 and related caspase activity. It is a biotinylated derivative of the Caspase-2 inhibitor Z-VDVAD-FMK (Cat # 1142). This conjugation with biotin allows detection of activated caspases in conjunction with avidin-tags. The inhibitor is designed as a methyl ester to facilitate cell permeability. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups).

Biotin-Val-Asp(OMe)-Val-Ala-Asp(OMe)-FMK

N/A

901.05

Caspase Inhibitor

Biotin-VDVAD-FMK, VDVAD-FMK, Biotin

C₄₀H₆₅FN₈O₁₂S

Liquid

DMSO (10 mM)

No

Yes

Single spot by TLC

N/A

-20°C

gel pack

12 months

Protect from light and moisture

cell

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Biotinylated

For cells, cell lines and tissues in culture till half confluency.