Human recombinant UBE2K

Catalog number
6432-3
Name
Human recombinant UBE2K
Size
3 nmol
Price
393.00 EUR
Supplier

Details

Background
UBE2K, also known as UbcH1 or E2-25K, is an E2 (ubiquitin conjugating enzyme) that is involved in the conjugation of ubiquitin to target substrates along with E1 and E3 enzymes. Once an E1 is carrying an activated ubiquitin in a ubiquitin-E1 thiolester complex, this activated ubiquitin is transferred to the active site cysteine of the E2 to form a ubiquitin-E2 thiolester complex. With the aid of an E3, the activated ubiquitin is then targeted to a specific substrate lysine. UBE2K is a biochemically important E2 due to its ability to form free K48-linked polyubiquitin chains in solution in the absence of an E3. This ability is attributed to UbcH1 possessing a C-terminal UBA domain, in addition to its E2 catalytic domain. UBE2K takes part in many cellular processes such as selective protein degradation, DNA repair, cell cycle control, and sporulation. In addition, UBE2K is involved in Alzheimer's disease, Huntington's disease and antigen processing through its interaction with amyloid-β, Huntington, and MHC-heavy chain proteins.
Concentration
N/A
Molecular weight
22.406 kDa
Synonym
Human recombinant UBE2K
Other name
HIP2, Huntingtin Interacting protein 2, HYPG, Ubiquitin-conjugating enzyme E2-25K kDa, Ubiquitin-protein ligase, Ubiquitin carrier protein, LIG, HIP-2, E2 (25K), DKFZp686J24237, EC 6.3.2.19.
NCBI gene number
3093
NCBI gene
UBE2K
Gene source
Human
NCBI number
P61086
Recombinant
Yes
Source
N/A
Purification
≥95%
Tested applications
RP-HPLC, N/A
Level of endotoxin
N/A
Tested activity
N/A
Biological activity
Typical enzyme concentration of 100 nM - 1 mM is used for in vitro conjugation, depending on conditions.
Reesults
N/A
Binding ability
N/A
Unit
N/A
Storage condition
-80°C
Shipping under
gel pack
Physical appearance
Liquid
Physical properties
75 µL of a 40 µM solution (3 nmoles) in TBS.
How to reconstitute
N/A
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants