Human CellExp™ IL-1beta, Human Recombinant

Catalog number
6460-10
Name
Human CellExp™ IL-1beta, Human Recombinant
Size
10 μg
Price
393.00 EUR
Supplier

Details

Background
Interleukin-1beta is produced by activated macrophages. IL-1beta stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL1beta proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. In comparison with the E. coli expressed protein, IL-1 beta is 50% more potent in promoting human CD4+ T cell differentiation into Th17 cells. This cytokine is produced in a serumfree, chemically defined media.
Concentration
N/A
Molecular weight
18 and 25 kDa, monomer, glycosylated
Synonym
Human Cellexp Human Recombinant IL-1beta
Other name
Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta.
NCBI gene number
3553
NCBI gene
IL1B
Gene source
Human
NCBI number
P01584
Recombinant
Yes
Source
Human 293 cell expressed
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Level of endotoxin
<1.0 EU per 1 μg of protein
Tested activity
N/A
Biological activity
ED₅₀ is typically 0.02 to 0.08 ng/mL. The specific activity was determined by the dose-dependent stimulation of the proliferation of mouse D10S cells.
Reesults
0.02 to 0.08 ng/ml
Binding ability
N/A
Unit
N/A
Storage condition
-80°C
Shipping under
gel pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from a PBS solution.
How to reconstitute
Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants