Human CellExp™ PECAM-1, Human Recombinant

Catalog number
7184-50
Name
Human CellExp™ PECAM-1, Human Recombinant
Size
50 μg
Price
382.00 EUR
Supplier

Details

Target
PECAM1
Background
PECAM is transmembrane glycoprotein that belongs to the Ig-related superfamily of adhesion molecules. It is highly expressed at endothelial cell junctions, and also expressed in platelets and in most leukocyte sub-types. The primary function of PECAM-1 is the mediation of leukocyte-endothelial cell adhesion and signal transduction. PECAM-1 has been implicated in the pathogenesis of various inflammation related disorders, including thrombosis, multiple sclerosis (MS), and rheumatoid arthritis. The human PECAM-1 gene codes for a 738 amino acid transmembrane glycoprotein containing a 118 amino acid cytoplasmic domain, a 19 amino acid transmembrane domain, and a 574 amino acid extracellular domain. Recombinant human PECAM-1 is a 572 amino acid glycoprotein comprising the extracellular domain of PECAM-1. Monomeric glycosylated PECAM-1 migrates at an apparent molecular weight of approximately 80.0-95.0 kDa by SDS-PAGE analysis under reducing conditions.
Concentration
N/A
Molecular weight
80-95 kDa
Synonym
Platelet endothelial cell adhesion molecule, CD31 antigen, EndoCAM
Other name
Platelet endothelial cell adhesion molecule, CD31 antigen, EndoCAM
NCBI gene number
5175
NCBI gene
PECAM1
Gene source
Human
NCBI number
P16284
Recombinant
Yes
Source
HEK 293 cells
Purification
≥97%
Tested applications
SDS-PAGE, HPLC
Level of endotoxin
<0.1 ng/μg of protein (
Tested activity
Determined by its ability to support the adhesion of activated Jurkat cells. The expected ED50 for this effect is 1.0 -1.5 μg/ml.
Biological activity
Determined by its ability to support the adhesion of activated Jurkat cells. The expected ED50 for this effect is 1.0 -1.5 μg/ml.
Reesults
N/A
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized powder
Physical properties
Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium phosphate, pH 7.5
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Aa sequence
ENSFTINSVD MKSLPDWTVQ NGKNLTLQCF ADVSTTSHVK PQHQMLFYKD DVLFYNISSM KSTESYFIPE VRIYDSGTYK CTVIVNNKEK TTAEYQLLVE GVPSPRVTLD KKEAIQGGIV RVNCSVPEEK APIHFTIEKL ELNEKMVKLK REKNSRDQNF VILEFPVEEQ DRVLSFRCQA RIISGIHMQT SESTKSELVT VTESFSTPKF HISPTGMIME GAQLHIKCTI QVTHLAQEFP EIIIQKDKAI VAHNRHGNKA VYSVMAMVEH SGNYTCKVES SRISKVSSIV VNITELFSKP ELESSFTHLD QGERLNLSCS IPGAPPANFT IQKEDTIVSQ TQDFTKIASK SDSGTYICTA GIDKVVKKSN TVQIVVCEML SQPRISYDAQ FEVIKGQTIE VRCESISGTL PISYQLLKTS KVLENSTKNS NDPAVFKDNP TEDVEYQCVA DNCHSHAKML SEVLRVKVIA PVDEVQISIL SSKVVESGED IVLQCAVNEG SGPITYKFYR EKEGKPFYQM TSNATQAFWT KQKASKEQEG EYYCTAFNRA NHASSVPRSK ILTVRVILAP WK
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants