Human CellExp™ CD200/OX-2, human recombinant

Catalog number
7384-50
Name
Human CellExp™ CD200/OX-2, human recombinant
Size
50 μg
Price
697.00 EUR
Supplier

Details

Target
CD200/OX-2
Background
CD200 also known as OX-2 membrane glycoprotein (OX-2), is a type-1 membrane glycoprotein, which contains two immunoglobulin domains (1 Ig-like C2-type (immunoglobulin-like) domain and Ig-like V-type (immunoglobulin-like) domain), and thus belongs to the immunoglobulin superfamily. CD200 / OX-2 is widely expressed in multiple cell types. CD200 interacts with a structurally related receptor (CD200R) expressed mainly on myeloid cells and is involved in regulation of macrophage and mast cell function. OX-2 / CD200 and CD200R associate via their respective N-terminal Ig-like domains. CD200 also plays an important role in prevention of graft rejection, autoimmune diseases and spontaneous abortion.
Concentration
N/A
Molecular weight
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 23.4 kDa. The predicted N-terminus is Gln 31. DTT-reduced Protein migrates as 35-45 kDa due to glycosylation.
Synonym
CD200, MOX1, MOX2, MRC, OX-2, My033
Other name
CD200, MOX1, MOX2, MRC, OX-2, My033
NCBI gene number
4345
NCBI gene
CD200
Gene source
Human
NCBI number
P41217
Recombinant
Yes
Source
HEK293 cells
Purification
≥98%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD200R1 at 2 μg/ml. The concentration of rhCD200 that produces 50% of the optimal binding response is approximately 0.5‑16 ng/ml.
Reesults
Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD200R1 at 2 μg/ml. The concentration of rhCD200 that produces 50% of the optimal binding response is approximately 0.5‑16 ng/ml.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants