Human CellExp™ COMP/TSP5, human recombinant

Catalog number
7452-10
Name
Human CellExp™ COMP/TSP5, human recombinant
Size
10 μg
Price
246.00 EUR
Supplier

Details

Target
COMP/TSP5
Background
Cartilage oligomeric matrix protein (COMP) also known as Thrombospondin-5 (TSP5), EDM1, EPD1, MED, PSACH, THBS5, which belongs to the thrombospondin family. COMP / TSP5 contain 4 EGF-like domains, 1 TSP C-terminal (TSPC) domain, 8 TSP type-3 repeats. Abundantly expressed in the chondrocyte extracellular matrix, and is also found in bone, tendon, ligament and synovium and blood vessels. COMP may play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. COMP can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Thrombospondin-5 could play a role in the pathogenesis of osteoarthritis. COMP is a marker of cartilage turnover.
Concentration
N/A
Molecular weight
This protein fused with 6×His tag at the C-terminus, has a calculated MW of 82 kDa. The predicted N-terminus is Gln 21. DTT-reduced Protein migrates as 110-130 kDa due to glycosylation.
Synonym
COMP, TSP5, EDM1, EPD1, MED, PSACH, THBS5.
Other name
COMP, TSP5, EDM1, EPD1, MED, PSACH, THBS5.
NCBI gene number
1311
NCBI gene
COMP
Gene source
Human
NCBI number
P49747
Recombinant
Yes
Source
HEK293 cells
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells, when rhCOMP / Thrombospondin‑5 immobilized at 1 µg/well. More than 45% of ATDC-5 cell adhesion will be induced.
Reesults
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells, when rhCOMP / Thrombospondin‑5 immobilized at 1 µg/well. More than 45% of ATDC-5 cell adhesion will be induced.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants