Human CellExp™ Osteoprotegerin / TNFRSF11B, human recombinant

Catalog number
7514-20
Name
Human CellExp™ Osteoprotegerin / TNFRSF11B, human recombinant
Size
20 μg
Price
213.00 EUR
Supplier

Details

Target
Osteoprotegerin / TNFRSF11B
Background
Tumor necrosis factor receptor superfamily member 11B (TNFRSF11B) is also known as Osteoclastogenesis inhibitory factor (OCIF), Osteoprotegerin (OPG). TNFRSF11B is a secreted homodimer protein, which can interact with TNFSF10 and TNFSF11. TNFRSF11B acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. TNFRSF11B inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.
Concentration
N/A
Molecular weight
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 44.4 kDa. The predicted N-terminus is Glu 22. DTT-reduced Protein migrates as 55 kDa due to glycosylation.
Synonym
TNFRSF11B, OCIF, OPG, Osteoprotegerin
Other name
TNFRSF11B, OCIF, OPG, Osteoprotegerin
NCBI gene number
4982
NCBI gene
TNFRSF11B
Gene source
Human
NCBI number
O00300
Recombinant
Yes
Source
HEK293 cells
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized rh TNFRSF11B at 10 μg/ml (100 μl/well) can bind human TNFSF11 Fc Chimera with a linear ranger of 4-256 ng/ml.
Reesults
N/A
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants