BRG1 bromodomain (1448-1575 aa) (GST-tagged), human recombinant

Catalog number
7650-100
Name
BRG1 bromodomain (1448-1575 aa) (GST-tagged), human recombinant
Size
100 μg
Price
697.00 EUR
Supplier

Details

Target
BRG1
Background
The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. BRG1 is a member of the SWI/SNF protein family, which forms part of a large ATP-dependent chromatin remodeling complex. This complex is required for transcriptional activation of genes normally repressed by chromatin. BRG1 is mutated in many cancer cell lines, such as breast, prostate, lung, pancreas and colon. Further, BRG1 has an important role as a tumor suppressor. This protein can be used for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Concentration
N/A
Molecular weight
41.8 kDa (1448-1575 aa + GST Tag)
Synonym
Human recombinant BRG1 bromodomain (1448-1575 aa) (GST-tagged)
Other name
Protein BRG-1, BAF190A Mitotic growth and transcription activator, BRG1-associated factor 190A, ATP-dependent helicase SMARCA4.
NCBI gene number
6597
NCBI gene
SMARCA4
Gene source
Human
NCBI number
P51532
Recombinant
Yes
Source
E. coli
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Level of endotoxin
N/A
Tested activity
N/A
Biological activity
N/A
Reesults
N/A
Binding ability
N/A
Unit
N/A
Storage condition
-80°C
Shipping under
dry ice
Physical appearance
Liquid
Physical properties
50 mM Tris, pH 7.5, containing 500 mM sodium chloride, 5% glycerol, and 5 mM β-mercaptoethanol.
How to reconstitute
N/A
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants