EZCut™ TEV Protease, Recombinant

Catalog number
7847-1000
Name
EZCut™ TEV Protease, Recombinant
Size
100 μg
Price
142.00 EUR
Supplier

Details

Target
TEV Protease
Background
BioVision’s EZCut™ TEV Protease is a cysteine protease that recognizes the cleavage site of Glu-Xaa- Xaa-Y- Xaa-Gln-(Gly/Ser) and cleaves between Gln and Gly/Ser. The optimal sequence is Glu-Asn-Leu-Tyr-Phe-Gln-Ser/Glycine (ENLYFQS/G). It contains an enhanced form of a catalytic fragment of the NIa protein of Tobacco etch virus (TEV). TEV Protease is a restriction grade protease that has robust activity at 4C with high specificity and great stability. The optimal temperature for cleavage with this enzyme is 34°C. The protease can be used for the removal of affinity tags from fusion proteins. It contains a C-terminal His tag and can be easily removed after cleavage reactions by passing the reaction through a Ni-chelating resin. BioVision’s EZCut™ TEV Protease is an improved version of TEV protease that is highly site-specific, highly active, and significantly more stable than native TEV protease, resulting in enhanced long-term activity.
Concentration
1 mg/ml
Molecular weight
28.6 kDa (2038–2279 aa + C-terminal poly-his tag).
Synonym
Nuclear inclusion protein A, NIa protein
Other name
Nuclear inclusion protein A, NIa protein
NCBI gene number
N/A
NCBI gene
N/A
Gene source
N/A
NCBI number
N/A
Recombinant
Yes
Source
E. coli
Purification
≥95%
Tested applications
SDS-PAGE, HPLC
Level of endotoxin
N/A
Tested activity
The EZCut™ TEV Protease has an activity of ≥10,000 units/mg.
Biological activity
The EZCut™ TEV Protease has an activity of ≥10,000 units/mg.
Reesults
≥10,000 units/mg
Binding ability
N/A
Unit
One unit is defined as the amount of EZCut™ TEV Protease required to cleave >90% of 10 µg a control protein substrate (containing an ENLYFQS sequence at N-terminus) in 1 h at 37°C.
Storage condition
-80°C
Shipping under
dry ice
Physical appearance
Liquid
Physical properties
1 mg/ml solution in 0.1 M Tris-HCl, 0.5 M NaCl, 20% glycerol, 5 mM DTT and 0.5 mM EDTA, pH 8.0
How to reconstitute
N/A
Aa sequence
2038–2279 aa
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Additional source
Recombinants or rec. proteins
Group
recombinants