EZCut™ SUMO Protease 1 (His-tagged), Yeast Recombinant

Catalog number
7868-500
Name
EZCut™ SUMO Protease 1 (His-tagged), Yeast Recombinant
Size
500 Units
Price
262.00 EUR
Supplier

Details

Target
SUMO Protease 1
Background
SUMO (Small Ubiquitin-like MOdifiers) Protease 1 (Ulp1, Ubl-specific protease 1 from Saccharomyces cerevisiae) is a highly active cysteine protease. It is highly specific as it recognizes the tertiary structure of the ubiquitin-like (UBL) protein, SUMO (Smt3), rather than its amino acid sequence. SUMO fusion tag, as an N-terminal fusion partner, has been shown to enhance functional protein production in prokaryotic and eukaryotic expression systems with significantly improved protein stability and solubility. BioVision’s Recombinant EZCut™ SUMO Protease 1 containing an N-terminal His-tag is expressed in E. coli and purified by proprietary chromatographic techniques. The EZCut™ SUMO Protease 1 can be used to cleave SUMO protein tag from recombinant SUMO-fusion proteins. The optimal temperature for cleavage is 30°C; however, the enzyme is active over wide ranges of temperature and pH. After the completion of the cleavage reaction, the protease can be easily removed from the reaction by affinity chromatography using the Ni chelating resin (Cat. # 6562-1, 10, 100, 500).
Concentration
0.1 mg/ml (100 U/µl)
Molecular weight
28.7 kDa (403-621 aa + N-terminal Poly-His tag).
Synonym
Ulp1 peptidase, SUMO Protease
Other name
Ulp1 peptidase, SUMO Protease
NCBI gene number
856087
NCBI gene
ULP1
Gene source
Yeast
NCBI number
Q02724
Recombinant
Yes
Source
E. coli
Purification
≥90%
Tested applications
SDS-PAGE , N/A
Level of endotoxin
N/A
Tested activity
The EZCut™ SUMO Protease 1 (His-tagged) has an activity of ≥ 1 X 106 units/mg.
Biological activity
The EZCut™ SUMO Protease 1 (His-tagged) has an activity of ≥ 1 X 106 units/mg.
Reesults
≥ 1 X 106 units/mg.
Binding ability
N/A
Unit
One unit is defined as the amount of EZCut™ SUMO Protease 1 required to cleave >90% of 5 µg a control protein substrate (SUMO-GFP) in 1 h at 37°C.
Storage condition
-80°C
Shipping under
Dry ice
Physical appearance
Liquid
Physical properties
0.1 mg/ml (100 U/µl) solution in 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT and 20% glycerol pH 8.0
How to reconstitute
N/A
Aa sequence
403-621 aa + N-terminal Poly-His tag
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Conjugation
histidine
Additional source
Recombinants or rec. proteins
Group
recombinants