Proteinase K 20mg/ml Solution

Catalog number
9211-5
Name
Proteinase K 20mg/ml Solution
Size
5 ml
Price
235.00 EUR
Supplier

Details

Synonyms
Endopeptidase K, Tritirachium alkaline proteinase
Alternates names
Protease K, Endopeptidase K, Tritirachium alkaline proteinase
Taglines
A serine protease which inactivates nucleases
Biomolecule/Target
NA
NCBI Gene ID #.
5621
NCBI Gene Symbol
PROK
Gene Source
N/A
Accession #
P06873
Recombinant
Yes
Source
Tritirachium album
Purity
N/A
Endotoxin Level
N/A
Activity (Specifications/test method)
>30 units/mg
Biological activity
N/A
Results
N/A
Molecular Weight
N/A
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
20 mg/ml
Appearance
Liquid
Physical form description
Recombinant Proteinase K dissolved in 50 mM Tris-HCl, 2 mM Calcium acetate, pH 8.0 to a final concentration of 20 mg/ml.
Reconstitution Instructions
N/A
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Gene
Proteinase K s a broad-spectrum serine protease. The enzyme was discovered in 1974 in extracts of the fungus Engyodontium album (formerly Tritirachium album).Proteinase K is able to digest hair (keratin), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. This enzyme belongs to Peptidase family S8. The molecular weight of Proteinase K is 28,900 Daltons (28.9 kDa).
Description
The volume (ml) in milliliters of buffered % w/v solutions at the medium pH are also used for DNA extraction Organic (Phenol-Chloroform) Extraction, Non-Organic (Proteinase K and Salting out), Chelex (Ion Exchange Resin) Extraction, EDTA or PBS aqueous.