Human CellExp™ Recombinant Ebolavirus BDBV Envelope Glycoprotein 1 (GP1)

Catalog number
P1061-50
Name
Human CellExp™ Recombinant Ebolavirus BDBV Envelope Glycoprotein 1 (GP1)
Size
50 μg
Price
403.00 EUR
Supplier

Details

Target
GP
Background
EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L). GP protein contains 160-kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.
Concentration
N/A
Molecular weight
31.8 kDa
Synonym
GP1, GP, Envelope glycoprotein, GP2 (subtype Bundibugyo,strain Uganda 2007)
Other name
GP1, GP, Envelope glycoprotein, GP2
NCBI gene number
9487265
NCBI gene
GP
Gene source
Bundibugyo virus
NCBI number
B8XCN0
Recombinant
Yes
Source
HEK 293 cells
Purification
> 95%
Tested applications
SDS-PAGE, N/A
Level of endotoxin
< 1.0 EU per/μg
Tested activity
N/A
Biological activity
N/A
Reesults
N/A
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.
How to reconstitute
Reconstitute in 1X PBS to the desired protein concentration.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants