D-(+)-Biotin for cell activation

Catalog number
9587-5G
Name
D-(+)-Biotin for cell activation
Size
5 g
Price
240.00 EUR
Supplier

Details

Description
D-(+)-Biotin is a coenzyme and a B vitamin. May be used to elute proteins from avidin/streptavidin resins.
Peptide sequence
N/A
CAS number
58-85-5
Molecular weight
244.31
Category
Biochemicals
Other name
D-Biotin, Bios II, Coenzyme R, Vitamin B7, Vitamin H
Molecular formula
C₁₀H₁₆N₂O₃S
Physical appearance
White to off-white solid
Supplied with
N/A
Is this a salt?
No
Is it cell-permeable?
No
Purification
≥98%
Reconstitute instructions
DMSO (>40 mg/ml)
Storage condition
+4°C
Shipping condition
RT
Maximum time for storage
36 months
Storage instructions
Protect from air and light
Tissue
cell
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation
Biotinylated
Additional description
For cells, cell lines and tissues in culture till half confluency.