Mca-RPPGFSAFK(Dnp) for cell activation

Catalog number
B1132-500
Name
Mca-RPPGFSAFK(Dnp) for cell activation
Size
500 µg
Price
142.00 EUR
Supplier

Details

Description
A useful fluorescence resonance energy transfer (FRET) substrate for assaying ECE-1 and ECE-2, ACE1 and ACE2. neprilysin, thermolysin (TLN), MMP-2 and MMP9, thimet oligopeptidase, cathepsin A, cathepsin X/Z, BACE, and insulinase, but not MMP1. Fluorescence of Mca (donor) is quenched by Dnp (quencher) in the intact peptide. Proteolytic cleavage of the FRET peptide separates the donor and the quencher, recovering the quenched fluorescence of Mca that is directly related to the enzyme activities and can be monitored at Ex/Em: 328 nm/393 nm
Peptide sequence
Mca-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(Dnp)-OH
CAS number
N/A
Molecular weight
1388.46
Category
Biochemicals
Other name
Protease substrate; Mca - RPPGFSAF - K(Dnp)
Molecular formula
C₆₆H₈₁N₁₅O₁₉ 
Physical appearance
White to off-white solid
Supplied with
N/A
Is this a salt?
TFA
Is it cell-permeable?
N/A
Purification
≥95% by HPLC
Reconstitute instructions
DMSO (>10 mg/ml)
Storage condition
-20°C
Shipping condition
Gel pack
Maximum time for storage
36 months
Storage instructions
Protect from air and light
Tissue
cell
Additional description
For cells, cell lines and tissues in culture till half confluency.