Caspase-3 Colorimetric Assay Kit

Catalog number
K106-400
Name
Caspase-3 Colorimetric Assay Kit
Size
400 assays
Price
955.00 EUR
Supplier

Details

Kit's description
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-3/CPP32 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.
Highlights
• Detection method: Absorbance (400 or 405 nm) • Species reactivity: Mammalian • Kit size: Convenient sizes (25, 100, 200, 400 assays) • Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Kit's benefits
• Simple one-step procedure; takes 1-2 hours • Fast and convenient • Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.
Category
Apoptosis Assay Kit
Kit's other name
none
Contents
• Cell Lysis buffer • 2X Reaction Buffer • DEVD-pNA (4 mM) • DTT (1 M) • Dilution Buffer
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
Mammalian
Applications
Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Samples tested
Cell and tissue lysates
Test
Biovision supplies other types of Assays as 1.
Description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.