EZCell™ Intracellular Nitric Oxide Synthase (NOS) Detection Kit

Nitric oxide synthases (EC 1.14.13.39) (NOSs) are a family of enzymes that catalyze the production of nitric oxide (NO) from L-arginine. Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. There are three isoforms of NOS: endothelial (eNOS), neuronal (nNOS), and inducible (iNOS). nNOS accounts for the production of NO in central nervous system, where NO participates in cell communication and information storage. eNOS produces NO in blood vessels and is involved with the regulation of vascular function. In contrast to other isoforms, iNOS is expressed de novo under oxidative stress conditions and produces large amounts of NO as a part of body’s defense mechanism. BioVision’s Intracellular Nitric Oxide Synthase Detection kit uses a dye that reacts with intracellular NO produced by NOS to produce fluorescence (Ex/Em = 485/530 nm), which is proportional to the concentration of intracellular NOS and can be detected using a microplate reader or a fluorescence microscope. This kit provides a simple, non-radiometric method for detection of intracellular NOS in the cells.

• Detection method: fluorescence microscope (Ex/Em = 485/530) • Applications: Detection of NOS activity in adherent cells. Screening/studying/characterizing stimulators/inhibitors that affect intracellular levels of NOS. • Kit size: 100 assays • Compatible samples: NOS producing cells/cell lines

• Simple one step staining procedure Takes only 1-2 hrs • Non-radiometric fluorescent detection

Oxidative stress, Metabolism

Cell Based Nitric Oxide Synthase (NOS) Detection Kit, eNOS assay, iNOS assay, nNOS assay

NOS Assay Buffer Staining Dye (In DMSO)

-20°C

gel pack

12 months

Mammalian cells

• Detection of NOS activity in adherent cells. • Screening/studying/characterizing stimulators/inhibitors that affect intracellular levels of NOS.

• NOS producing cells or cell lines.

The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.