ADPsensor™ Universal Kinase Activity Assay Kit

Catalog number
K212-100
Name
ADPsensor™ Universal Kinase Activity Assay Kit
Size
100 assays
Price
540.00 EUR
Supplier

Details

Kit's description
Protein Kinases belong to a large family of phosphotransferases that mediate a wide spectrum of metabolic events via signal transduction pathways. Protein Kinases catalyze transfer of a phosphate group from a phosphate donor to a substrate protein. Kinases modulate the function of numerous proteins and are well-known therapeutic targets in many diseases like cancer, inflammation and diabetes etc. BioVision’s ADPsensor™ Universal Kinase Assay Kit is based on the fluorescence detection of ADP generated by Kinase activity. In this assay, ADP formed during the kinase reaction is converted to an intermediate by the ADP Sensor Mix, which then reacts with a probe to generate a strong and stable fluorescence signal at Ex/Em = 535/587 nm. ATP in the kinase reactions doesn’t interfere with the assay; therefore our kinase assay can be read kinetically (or at endpoint). The kit provides a universal tool to measure kinase activity in presence of substrates of your interest, or to screen/characterize kinase inhibitors. The assay is simple, non-radioactive, highly sensitive (
Highlights
Ultrasensitive, Simple, HTP adaptable fluorometric assay kit for Kinase activity measurement. Can detect as low as 1 ng of active Kinase. Detection method: Fluorometric (Ex/Em = 535/587 nm). Applications: Detection of Kinase activity, Characterization of Kinase substrates and inhibitors. Sample Type: Purified/Enriched Kinases, Tissue or cell extracts.
Kit's benefits
• Ultrasensitive, Simple, HTP adaptable fluorometric assay kit for Kinase activity measurement. • Can detect as low as 1 ng of active Kinase. • Free of ATP interference (up to 1 mM)
Category
Metabolism
Kit's other name
none
Contents
Kinase Assay Buffer ADP Sensor I ADP Sensor II Probe (in DMSO) Ultra-pure ATP (ADP depleted) DTT (1M) ADP Standard
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
Universal
Applications
Detection of Kinase activity, Characterization of Kinase substrates and inhibitors.
Samples tested
• Purified/semi-purified/immuno-precipitated Kinases • Crude cell/tissue extract
Test
Biovision supplies other types of Assays as 1.