Mitochondria/Cytosol Fractionation Kit

Catalog number
K256-25
Name
Mitochondria/Cytosol Fractionation Kit
Size
25 assays
Price
227.00 EUR
Supplier

Details

Kit's description
The Mitochondria/Cytosol Fractionation Kit provides unique formulations of reagents for effective isolation of a highly enriched mitochondrial fraction from cytosolic fraction of mammalian cells including both apoptotic and nonapoptotic cells. The enriched mitochondrial and cytosolic fractions can be used for studying apoptotic and signal transduction pathways to detect translocation of factors interested between the two fractions by Western blotting, ELISA, or other assays. Procedures are simple and easy to perform, no ultracentrifugations and toxic chemicals are involved.
Highlights
• Detection method: Western blotting, ELISA, or other assays. • Species reactivity: Mammalian • Kit size: 25, 100 assays • Applications: Effective isolation of a highly enriched mitochondrial fraction from cytosolic fraction of mammalian cells including both apoptotic and nonapoptotic cells. .
Kit's benefits
• Simple procedure; takes only 3-4 hours • Fast and convenient • The fractionation procedure is simple and staightforward. No ultracentrifugations are required. No toxic chemicals are involved.
Category
Apoptosis Assay Kit
Kit's other name
none
Contents
• Mitochondria Extraction Buffer • 5X Cytosol Extraction Buffer • DTT (1 M) • Protease Inhibitor Cocktail
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Effective isolation of a highly enriched mitochondrial fraction from cytosolic fraction of mammalian cells including both apoptotic and nonapoptotic cells. .
Samples tested
Cells and tissues